Using The Low Pressure Gradient Mode; Using The High Pressure Gradient Mode; Direct Control Of The Pump; Maximum Pressure - Knauer WellChrom HPLC Pump K-1001 Handbuch

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Using the Low Pressure Gradient Mode

Using the High Pressure Gradient Mode

Direct Control of the Pump

Maximum Pressure

SOP 6
SOP 7
SOP 8
If you want to control up to three additional HPLC Pumps K–1001 for a high
pressure gradient, you can connect the appropriate number of pumps using the
serial RS232 interfaces according to „Using the RS232 Serial Interfaces in the
KNAUER-Net" on page 30.
Activate „GRADIENT MODE" in the SETUP menu, see „Using the SETUP
Menu" on page 12 and select individual, unique names from HPG A to HPG D
for all pumps connected. The number of pumps in your HPG system can be
selected by using the ON option in the second line. Only the pumps selected
with ON will be used for calculating the percentage composition in a program.
Every pump automatically takes control over all pumps connected, if execution
of a program is started on that pump.
If you want to use the Direct Control Mode you have to specify:
Maximum system pressure
Flow Rate and
Solvent Composition if working in LPG or HPG gradient mode
In addition to the maximum pressure specified in the setup menu, see „Using
the SETUP menu" on page 12 by either the pump head or the global pressure
limit, you can specify a maximum system pressure for the currently active mode
of operation or the currently active program.
Setting the Maximum Pressure
In addition to the maximum pressure specified in the SETUP menu, either by
the pump head or by the individually set pressure limit, you can specify a
different, lower system pressure for the currently active program.
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X.
1. Move the cursor to the parameter PRESSURE, see {1.4} in Fig. 2 on
page 8.
2. Type in the desired value for the maximum pressure. The selected value
is marked with two vertical lines which indicate that this is a programmed,
not a measured value.
3. Press any arrow key for confirmation.
Setting the Flow Rate
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X. Use this
SOP for setting the correct flow rate.
1. Position cursor on field FLOW, see {1.3} in Fig. 2 on page 8.
2. Enter the desired flow rate with the numeric keys.
3. Press any arrow key for confirmation.
Setting the Solvent Composition
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X, when
working in any gradient mode.
1. Move the cursor to the field % A, see {1.8} in Fig. 2 on page 8.
Operating the HPLC Pump K–1001