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Operating The Hplc Pump K-1001; Working Example For Setting Parameters; Selection Of Operation Mode - Knauer WellChrom HPLC Pump K-1001 Handbuch

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Operating the HPLC Pump K–1001
Operating the HPLC Pump K–1001

Working Example for setting parameters

SOP 4
SOP 5
Fig. 13
You need to setup your pump prior to operation. Use the information in sections
„Menu Structure of the HPLC Pump K–1001" on page 11,
„Using the SETUP Menu" on page 12,
For your convenience, this example points out how to configure a binary low
pressure gradient using valves A and B.
This example may not comply with your needs and is given for
convenience only. You need to select parameters that meet your
requirements. Using this example without proper adjustment of
parameters may lead to damage of your equipment.
Enter the SETUP menu, see Fig. 8 on page 13.
Selection of correct pump head:
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X.
1.
Select menu PUMP HEAD by pressing
2.
Use
or
to scroll through the list of pump heads until the installed type
of pump appears, e.g. 10 ml, steel. Selection of a pump head
automatically adjusts proper absolute maximum system pressure.
3.
Press
to confirm selection and return to the rhombus.

Selection of operation mode:

This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X.
1.
Return to page GRADIENT MODE by pressing
2.
Press
and then
LPG appears.
3.
Use
to position cursor in the second line.
4.
Select ON or -- (off) at positions A, B, C and D for the solvent channels in
either mode you wish to use. The number of ONs selected refers to all
programs in the pumps memory.
5.
At any position you can use
6.
Position cursor on next position, e. g. B and select ON .
7.
Select three times
8.
The display should appear as shown in Fig. 13.
9.
Leave this menu page with
Load
Edit Prog
Prog
Clear 0
GRADIENT MODE:
Link
View
Hold 0
Run 1
Example for a gradient setup with solvents A and B in all programs
to scroll through the gradient mode options until
or
to select ON.
to position cursor at the rhombus.
to return to the main menu.
Flow
Pressure
Events
[ml/min]
[0.1 Mpa]
off 0
LPG
ON
ON
Time
%A
%B
[min]
.
on 1
2
Setup
GLP
--
--
%C
%D
17

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