Werbung
Verfügbare Sprachen
Verfügbare Sprachen
20
Piston Backflushing
SOP 12 Preparing your HPLC Pump K–1001 for piston backflushing
Fig. 15
Open the „De-aeration screw for opening purge valve", Pos. {4.3} prior to
starting the purging function to avoid damaging your column system.
1.
Purging without opening the purging outlet may cause damage to your
column and system.
2.
Press purge key {2.2}, see Fig. 2 on page 8, for start and stop of purging.
In LPG mode, the pump is purged with the currently active solvent
composition.
3.
Select a flow rate for purging, see SOP 7 „Setting the Flow Rate" on
page 18.
4.
In LPG mode, purging can be performed either in mode MIX, using the
currently active solvent composition or separately for each chammel A to
D. Select the desired mode by positioning the cursor on the appropriate
positions MIX, A, B, C or D.
Backflushing the piston removes traces of salt and other decontaminants from
the backside of the pistons.
Especially if you use saline solvents or buffer solution we highly recommend
that you continuously backflush in order to prevent crystallization which can
damage your piston seals.
For best protection and error free long time operation of the pump head it is
recommended always to backflush the piston with an appropriate solvent
(mostly water) according to the following SOP.
This SOP applies to the HPLC Pump K–1001
1.
Push a 1/16" ID tube over both piston backflushing capillaries.
Piston backflushing capillaries
2.
Place one low end of the tubing in a flask.
3.
Fill the priming syringe with the backflushing liquid.
4.
Connect the syringe with the other tubing.
5.
Press liquid through the pump head, until it flows without any air bubbles
into the container.
6.
Remove tubings from capillary opening.
If you want operation with continuous backflushing you can attach two
containers of rinsing liquid instead of the priming syringe. The containers should
be positioned that one container is located higher than the other, thus ensuring
liquid flow through the pump head without any assistance.
When not backflushing continuously, we recommend to connect both capillary
openings with a tubing to prevent vaporization of solvents and drying out of the
piston chamber.
Operating the HPLC Pump K–1001
Werbung
Kapitel
