Fluorescence - Leica DM1000 Bedienungsanleitung

9. Contrast Methods
• If necessary:
Insert the λ or λ/4 compensator into the filter
holder integrated in the condenser holder and
rotate to the left, roughly as far as the stop.
CLP/PH condenser:
Insert the λ or λ/4 compensator into the slot
on the side of the condenser.
Condensers UCLP and UCA/P:
Rotate the condenser disk into position λ or
λ/4.
Alternative:
4x20 mm compensators can be pushed into
the compensator slit.
Fig. 49 Leica DM1000 with
Fluorescence illuminator and 106z lamphousing
46

9.2 Fluorescence

• Insert a suitable specimen and rotate an
appropriate objective into place.
• Focus the image initially in transmitted light if
appropriate.
• Switch on the incident light source at the
external power unit.
• Open the shutter.
• Select an appropriate fluorescence filter
cube.
• Switch magnification changer*, if present, to
factor 1x.
• Disengage the BG 38 filter if there is no
disturbing red background. Always engage
the filter for photography, however.
• Open the aperture diaphragm.
• Open the field diaphragm until the whole field
of view is just illuminated.
If necessary center the field diaphragm.