Principle Of The Procedure; Contents Of The Kit; Additional Instrumentation Required; Type Of Specimens, Handling And Storage - Axis-Shield RPR Bedienungsanleitung

carbon, which enhances the visual difference between a positive and a negative result. When these
antibodies are detected in serum or plasma it is a presumptive diagnosis for syphilis.
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Principle of the procedure

Axis-Shield RPR is a macroscopic non-treponemal flocculation test and it is used to detect reagin.
When a drop of positive serum and reagent are mixed on a test slide, black flocculants are formed
which are visible macroscopically due to the presence of the carbon particles. When reagin antibody is
not present, flocculation does not occur and the appearance is that of an even grey colour.
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Contents of the kit

Component Description
5 x 2 ml
2 x 1 ml
2 x 1 ml 5 x
50 x
500 x
2 x
500 x
1 x
1 x
5

Additional instrumentation required

Platform mixer, capable of operating at 100 rpm.
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Type of specimens, handling and storage

Human serum or plasma may be used. No special preparation or fasting of the patient is necessary.
Blood should be collected by normal venipuncture technique and handled with the proper precautions
e.g. according to the NCCLS. H3-A4*, and OSH-A FDA 29cfr 1910.1030**.
* NCCLS. H3-A4, Procedures for the Collection of Diagnostic Blood Specimen by Venipuncture;
Approved Standard [ISBN 1-56238-350-7].
** OSH-A FDA 29cfr: Blood borne pathogens. 1910.1030 Occupational Safety and Standards.
Fresh specimens may be stored for up to one week at 2 to 8 °C if free of microbial contamination. If
longer storage is required, specimens should be stored frozen at –20 °C or below. Conditions that
favor microbial growth should be avoided. The quality of the specimens can be seriously affected by
microbial growth, which may lead to erroneous results.
Antigen Suspension
Modified VDRL antigen
Preservative:
Sodium azide 1 mg/ml.
Ready to use as supplied.
NB:WARNING
Negative Control
Antigen-Suspension Human serum. Preservative:
Sodium azide 1 mg/ml. Ready to
use as supplied.
Positive Control
Diluted human serum reactive for Antigen-Suspension
anti-T.pallidum.
Preservatives: Sodium azide
1 mg/ml. Ready to use as
supplied.
Disposable test cards Each card
contains 10 test circles
Disposable plastic pipettes
Suction caps for pipettes
Disposable plastic spatulas
Plastic dispensing bottles
Dispensing needles
Specimen collection
Specimen handling and storage
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H319;
EUH032;
P264-280
P305+351
+338
P337+313
EUH032
EUH032