Cleaning And Sterilization - HTL discovery comfort DV8-10 Handbuch

• Damage to the piston or seal due to prolonged use
with the aggressive liquids.
Disassemble the pipette as described above.
Replace the piston, seal and O-ring (see Section 12).
Rinse the inside of the shaft in distilled water and dry.
Lubricate the seal and O-ring with the lubricant, that
has been included with each pipette.
The replacement of the piston requires conducting
of calibration procedure.
Note: The parts of DV2/D2 and DV/D10 pipette
should be lubricated evently with a minimum
amount of lubricant.
• The pipette is reassemled improperly.
Disassemble the pipette and reassemble it, observ-
ing the proper sequence of steps (Fig. 3).
• No lubricant on the sealing elements.
Remove the tip ejector. Unscrew the shaft nut,
remove the shaft, piston assembly, seal and O-ring.
Rinse the removed parts in distilled water and dry
thoroughly. Lightly lubricate the inside surfaces of
the seal and the O-ring with the included lubricant.
Reassemble the pipette in the reverse order.
• Contamination of the inside of the pipette caused by
extensive aspiration of chemically aggressive liquids or
because liquid got inside the pipette.
Remove the tip ejector. Unscrew the nut, remove
the shaft, piston assembly, seal and O-ring. Rinse
the removed parts with distilled water and dry thor
oughly. Lightly lubricate the inside surfaces of the
seal and the O-ring with the lubricant. Reassemble
the pipette in the reverse order.
If you find an increase in the pipetting force, which
could happen after repetitive autoclaving of the pipette:
Remove the tip ejector. Unscrew the shaft nut, and
then remove the shaft, piston assembly, seal and
O-ring. Rinse the removed parts in distilled water and
dry. Lubricate the internal surfaces of the seal and
O-ring with lubricant that has been included with each
pipette. Reassemble the pipette in opposite order.
Note: All parts of the pipette can be autoclaved (see
Section 10)
The shaft of the 5000 and 10000 models should be
autoclaved without the filter.
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If the problem continues after carrying out the above
steps, contact your HTL representative.
Before returning the pipette, please ensure that the
pipette is completely free of any chemical, radioactive or
microbiological contamination which could pose a threat
during transport and repair. As far as it is possible, clean
the pipette.

10 - CLEANING AND STERILIZATION

Cleaning
External surfaces of the pipetting pushbutton, the ejector
pushbutton, the handgrip, the shaft nut and the adjust-
ment knob may be cleaned using a cloth dampened in
isopropyl alcohol. The remaining parts removed from the
pipette during pipette disassembly may be washed with
distilled water or isopropyl alcohol.
Sterilization:
The pipette can be sterilized in the autoclave at 121°C for
20 minutes. After sterilization, the pipette should be dried
and cooled to room temperature.
It is recommended:
- to sterilize the pipettes in autoclave with an initial vacu-
um and drying cycle,
- prior to sterilization unscrew the shaft nut slightly in the
DV/D2-DV/D1000 pipettes, and unscrew the shaft
slightly in the DV/D5000 and DV/D10000. After autoclav-
ing these parts should be screwed tight again.
- to set the locking ring in lower (unlocked) postion prior
to sterilization
The precision of the results should not alter if the pipet-
ting process and autoclaving are carried out as
described in this manual. Because a slight change in the
accuracy of the dosage may occur, it is recommended to:
- check the calibration of the pipette after the initial first,
third and fifth autoclaving cycles and then after every 10
autoclaving cycles.
ENGLISH
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