Cleaning And Sterilization; Pipette Kit - HTL discovery comfort DV8-10 Handbuch

water density coefficient [μl/mg], which depends on tem-
perature and pressure according to the following table:
Temperature [°C]
20
21
22
23
24
25
If the average aspirated volume exceeds the permissible
value, the following should be done:
• Remove the pipetting pushbutton, (Fig. 4A),
Warning: The pipetting pushbutton consists of 2 parts:
the knob (Fig. 1A2) and the pushbutton (Fig. 1A1). After
removal of the pushbutton, both parts are separated.
• Holding the volume setting knob to protect it against
rotation, insert the calibration key into the cuts of the
calibration screw, (Fig. 4B),
• Turn the key clockwise to reduce the aspirated volume,
or counter-clockwise to increase the volume. One full
turn of the calibration key changes the pipette aspira-
tion volume by the amount given in the table, (Fig. 4C),
• Take out the key and fix the pipetting pushbutton
(Fig. 4D). The pipetting pushbutton should be fixed by
placing the knob on the arbor first (Fig. 1A2) and then
the pushbutton (Fig. 1A1).
Determine the average aspirated volume. The average
volume should be within the permissible range given in
the table. If the volume exceeds the values stated, the
recalibration procedure should be repeated.
More information on calibration procedure can be found
on www.htl.com.pl
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Pressure [kPa]
95.0 101.3 105.0
1.0028 1.0029 1.0029
1.0030 1.0031 1.0031
1.0032 1.0033 1.0033
1.0034 1.0035 1.0036
1.0037 1.0038 1.0038
1.0039 1.0040 1.0040

6 - CLEANING AND STERILIZATION

Cleaning
External surfaces of the pipetting pushbutton, the ejector
button, the handgrip, shaft and the adjustment knob may
be cleaned using a cloth dampened in isopropyl alcohol.
Sterilization:
The pipette can be sterilized in the autoclave at 121°C for
20 minutes. After sterilization, the pipette should be dried
and cooled to room temperature.
It is recommended:
- to sterilize the pipette in autoclave with an initial vacuum
and drying cycle,
- prior to sterilization to unscrew the nut connecting the
handle and module slightly. After autoclaving these
parts should be screwed tight again.
- to set the locking ring in lower (unlocked) position prior
to sterilization.
The precision of the results should not alter if the pipetting
process and autoclaving are carried out as described
in this manual. Because a slight change in the accuracy
of the dosage may occur, it is recommended to:
- check the calibration of the pipette after the initial first,
third and fifth autoclaving cycles and then after every 10
autoclaving cycles.

7 - PIPETTE KIT

The pipettes are delivered in the kits including:
• Pipette
• Instruction manual
• Calibration key
• Pipette stand
• Identification labels
The stand assembly diagram is shown in Fig. 6.
ENGLISH
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