Sterilization; Troubleshooting - HTL DISCOVERY PRO Bedienungsanleitung

Descriptions of the parts are given in fig. 1, 6, 7 and 8.
Fig. 6, 7 and 8 can be found in section 8.
• While holding the Ejector unscrew the Shaft from the
handle (fig. 1 I),
• While holding the Ejector bush N, unscrew Ejector K
Then proceed depending in the pipette type:
2-100 μl pipettes (fig.6)
It is not necessary to disassemble the entire set to
inspect and clean the shaft. Unscrew the Shaft B from
the Shaft holder set C, remove the Seal D and the O-ring E.
To clean other elements:
• Unscrew the Shaft cap J,
• Remove from the Shaft the Plunger A, the Pipetting
spring I, the Blocking bush H, the Compression spring
G and the Compression bush F,
• Inspect the elements and proceed according to the
recommendations;
• Replace elements if they are damaged.
CAUTION: While disassembling, behave caution to pre-
vent the plunger from coming off due to the pipetting
spring.
200 μl pipettes (fig. 7)
• Unscrew the Shaft cap J,
• Remove from the Shaft the Plunger A, the Pipetting
spring I, the Blocking bush H, the Compression spring
G, the Compression bush F, the Seal D and the O-ring E,
• Inspect the elements and clean them according to the
recommendations,
• Replace elements if they are damaged.
CAUTION: While disassembling, behave caution to pre-
vent the plunger from coming off due to the pipetting
spring.
1000 μl pipettes (fig. 8)
• Unscrew the Shaft cap J,
• Remove from the shaft the Plunger A, the Compression
spring G, the Compression washer M, the Seal D and
the O-ring E.
Reassemble the elements in the reverse order. After
cleaning the plunger, apply a thin film of lubricant on its
surface. The lubricant is delivered with every pipettes.
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CAUTION: Recalibrate the pipette after replacing the
plunger. If the pipette does not work properly, check
whether it has been reassembled correctly.

6.2 STERILIZATION

The pipette can be sterilized in the autoclave at the tem-
perature of 121°C for 20 minutes. After sterilization, the
pipette should be dried and cooled down to room tem-
perature. It is recommended to sterilize the pipettes in
autoclave with initial vacuum and drying cycle.
CAUTION: Before using cleaning agents other than
those recommended by the manufacturer, check the
compatibility charts and consider chemical resistance of
the following plastics: PP, PC, POM, PA, PPS, PVDF
used to produce the pipette parts.
CAUTION:
- Sterilization in other conditions may cause the damage
of the pipette,
- Set the volume adjustment knob in its upper (unlocked)
position before sterilization.
Repeatability of obtained results does not change if the
pipette is operated properly and the autoclaving proce-
dure is conducted appropriately. As there can be a slight
change in the dosing accuracy it is recommended:
- To check calibration of the pipette after 1st, 3rd and 5th
autoclaving procedures, and every 10 autoclaving
cycles during further operation of the pipette.
The pipettes are UV resistant which was confirmed by
our tests. The recommended distance from the radiation
source to exposed element should be not less than
50 cm. Prolonged and very intense UV exposure can
cause de-coloration of pipette parts, without affecting its
performance.

7. TROUBLESHOOTING

If you notice an improper pipette operation, identify the
cause and eliminate the fault. To do this, follow the
instruction in the sequence provided. Replacement of
parts should be required only occasionally, and should
not occur under normal pipette use.
ENGLISH
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