HTL discovery comfort DV8-10 Handbuch Seite 4

If above described method of ejector adjustment is not
sufficient or the diameter of the ejector opening is too
large to eject the tip it is necessary to put the ejector cap
"M" onto the ejector, (Fig. 6C).
- in 2 and 10 μl pipettes (Fig. 6D)
The 2 μl - 10 μl pipettes may require the use of an ejector
cap "M" to efficiently eject certain brands of pipette tips.
Simply place the cap, supplied with the pipette, on the
bottom of the pipette shaft and slide the cap upwards
until it surrounds the bottom of the tip ejector.
The DISCOVERY Comfort is a high quality instrument
which offers excellent accuracy and precision.
The accuracy and precision (repeatability) of liquid
volume depend on the quality of tips used. The values
for accuracy and precision given in the table below were
obtained using HTL tips. Only those tips guarantee
correct operation of the pipettes and ensure accuracy
and precision of liquid sampling.
Volume Accuracy
Model Cat. no.
[μl] [%]
0.2 ± 12.0
DV2 4041
1.0 ± 2.7
D2 4051
Max 2.0 ± 1.5
Min 0.5 ± 4.0
DV10 4042
5.0 ± 1.0
D10 4052
Max 10.0 ± 0.5
Min 2 ± 3.0
DV20 4043
10 ± 1.0
D20 4053
Max 20 ± 0.8
Min 5 ± 2.5
DV50
4047
25 ± 1.0
D50 4057
Max 50 ± 0.8
Min 10 ± 1.6
DV100 4044
50 ± 0.8
D100
4054
Max 100 ± 0.8
Min 20 ± 1.2
DV200 4045
100 ± 0.8
D200 4055
Max 200 ± 0.6
Min 50 ± 1.0
DV250 4040
125 ± 0.8
D250 4050
Max 250 ± 0.6
Min 100 ± 1.6
DV1000 4046
± 0.7
500
D1000 4056
Max 1000 ± 0.6
Min 1000 ± 0.6
DV5000 4048
2500 ± 0.6
D5000 4058
Max 5000 ± 0.5
Min 1000 ± 2.5
DV10000 4049
5000 ± 0.8
D10000 4059
Max 10000 ± 0.5
3
Precision Fit to tips Shafts color
[%] μl for D
± 6.0
± 1.3
± 0.7
10 red
± 2.8
± 0.6
± 0.4
± 1.5
± 0.5
± 0.3
± 2.0
± 0.6
± 0.4
200 yellow
± 0.80
± 0.24
± 0.20
± 0.60
± 0.25
± 0.20
± 0.4
300 green
± 0.3
± 0.3
± 0.40
1000 blue
± 0.20
± 0.15
± 0.25
5000
± 0.20
± 0.15
white
± 0.6
± 0.3 10000
± 0.2
The accuracy and precision are obtained with HTL tips,
using a gravimetric method, performing at least 10 mea-
surements of distilled water at a temperature of 20±1°C,
according to EN ISO 8655 standard.
The pipette design enables the user to perform the
recalibration process according to the rules presented in
section 8.
2 - SETTING THE VOLUME
The volume display shown by the counter has three dig-
its, which should be read from top to bottom. In addition
the lowest counter drum is printed in a scale which
enables the setting of the volume in the minimum gradua-
tion range. Examples of the meanings of the black and
red digits:
Pipettes DV2, D2
Red figures at the bottom = 1/100 μl
Increment = 0.002 μl
Pipettes DV10, DV20, D10, D20
Red figures at the bottom = 1/10 μl
Increment = 0.02 μl
Pipettes DV50, DV100, DV200,
DV250, D50, D100, D200, D250
Black figures only = 1 μl
Increment = 0.2 μl
Pipettes DV1000, DV5000, D1000, D5000
Red figures at the top - ml
Increment =
Pipettes DV10000, D10000
Red figures at the top - ml
Increment = 20 μl
The volume of the pipette is set by the knob in the pipet-
ting pushbutton (Fig. 1A2) or by the black adjustment
knob (Fig. 1B). The volume adjustment can be performed
when the locking ring is set in the lower position (Fig.5A).
ENGLISH
2 μl 10 μl
4