Sectioning Instructions - Thermo Scientific Microm HM340E Benutzerhandbuch

Sectioning
Instructions
Conditions of Knife/Blade
Setting the Cutting angle
Thermo Fisher Scientific
Chapter 4
Working with the Microtome
h
azard of hand injuries
Due to moving parts on the microtome knife or blade, danger can
occur, which might lead to hand injuries if the safety features of the
microtome and the instruction manual are not complied with.
The knife or blade carriers of the microtome are easy to use; they can
be moved sideways and are equipped with knife guards for user safety
while adjusting knife or blade and specimen.
Note!
To cut usable sections, the following points are of utmost importance:
ONLY USE A SHARP KNIFE!
Edge • If the cutting edge is blunt, move the knife/blade horizontally either
to the right or left side to continue working with the sharp area of
the cutting edge, or have the knife re-sharpened or replace the used
blade by a new one.
• For optimal sectioning, front and back of the knife must be clean.
• Especially paraffin waste must be removed thoroughly!
Angle of the knife [a] in relation to the block surface [b]:
1 Clearance angle a
2 Wedge angle b
3 Upper sharpening
angle g
4 Lower sharpening
angle d
5 Blade angle e
6 Angle of inclination j
Working with the Microtome

Sectioning Instructions

[b]
Operation Manual HM340E
[a]
53