Checking Phase Contrast Rings - Leica DMI3000B Bedienungsanleitung

Leica DMI4000 B and Leica DMI6000 B:
Suitable aperture and fi eld diaphragm values have
been preset for each objective. The inci dent light
module has also been centered at the factory.
However, it may be necessary to readjust the
in cident light module in some cases after trans-
porting and setting up the stand. Therefore,
check the fi eld diaphragm centering.
The following procedure is provided for the inci-
dent light-bright fi eld illumination.
• Select an objective with moderate magnifi ca-
tion (10x–20x).
• Activate the incident light axis with the TL/IL
button (84.1).
• Press the IL-BF / Fluo button to activate the
bright fi eld contrast method (one of the vari-
able function buttons on the stand).
• Place a specimen on the stage.
• Focus the specimen using the SmartMove or
the focus wheels.
• Adjust the light intensity with the INT buttons
(84.3).
Adjusting the fi eld diaphragm*
• Close the fi eld diaphragm with the FD button
(84.4) or manually until the edge of the dia-
phragm (round or rectangular) appears in the
fi eld of view.
• If the limits of the fi eld diaphragm are not in the
center of the fi eld of view, move the posi tion of the
fi eld diaphragm to the center with the two center-
ing screws (92.1) on the right side of the stand.
• Use the function buttons FD (84.4) to open the
fi eld diaphragm to the point that they just dis-
appear from the fi eld of view.
* not in combination with structured illumination
• We recommend the use of a rectangular fi eld
diaphragm when using a digital camera. Match
the size of the diaphragm to the chip size of the
camera.

7.7 Checking Phase Contrast Rings

If your microscope is equipped for phase con-
trast, light rings to match your objectives will be
installed in the condenser.
The light rings are already centered in the fac-
tory. As a result of transport and setup of the
stand, however, in some cases centering maybe
be required again. Therefore check the center-
ing.
Note:
Each objective has its own light ring assigned to
it in the condenser. The test must therefore be
performed for each objective.
Regular phase contrast with phase objectives
When choosing an objective suitable for phase
contrast, the appropriate light ring is selected
automatically when using a motorized con-
denser. Otherwise, select the light ring manually.
Fig. 92
Adjusting the fi eld
diaphragm (incident light-
fl uorescence)
1 Adjusting screws for
moving the fi eld
diaphragm
7. Start-up
1
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