Basics Of Solid Phase Extraction (Spe) - MACHEREY-NAGEL CHROMABOND Gebrauchsanleitung

Basics of Solid Phase Extraction (SPE)

Solid phase extraction (SPE) is a powerful meth-
od for sample preparation and is used by most
chromatographers today. For more than 30 years
MACHEREY-NAGEL offers CHROMABOND
cartridges based on silica and polymeric materials.
In modern analyses SPE fulfills three important
tasks:
Enrichment of analytes
n n
up to factor 10,000 – increase of chromatographic
sensibility / improved limits of detection
Removal of interfering compounds
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Protection of subsequent analyses like HPLC, GC,
TLC, MS, UV, or IR spectroscopy...
Changing of an analyte's matrix
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Matrix which is simpler or more suitable for subse-
quent analyses
22 www.mn-net.com
Advantages of SPE compared to classical
liquid-liquid extraction:
Lower consumption of solvents
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SPE
®
Faster – enormous time savings
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Lower costs per sample
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Potential for automation
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High consistency in individual sample handling
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More specific selectivity because of the broad
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range of adsorbents and different retention
mechanisms
Optimization of extraction by variation or adjusting
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of the solid phase and chromatographic condi-
tions
Separation of food additives
2
1
5
4
3
after SPE
Time [min]
1. Acesulfam K
2. Saccharin
3. Benzoic acid
4. Sorbic acid
5. Aspartame
prior to SPE