Pipette Operation; Safety Label; Pipetting Techniques - Thermo Scientific Finnpipette Bedienungsanleitung

SETTING THE DELIVERY VOLUME
1. Set the delivery volume using the push button on the top of the pipette.
To increase the delivery volume, turn the push button counterclockwise.
To decrease the delivery volume, turn it clockwise.
2. Make sure that the desired delivery volume clicks into place and that
the digits are completely visible in the display window.
3. Do not set volumes outside the pipette´s specified volume range.
Using excessive force to turn the push button outside the range may jam
the mechanism and eventually damage the pipette.
TIP EJECTION
To help eliminate the risk of contamination, each pipette is fitted with a tip
ejector system.
The tip ejector system consists of a soft-touch tip ejector and specially designed
gearing mechanism. To release the tip, point the pipette at suitable waste
receptacle and press the tip ejector with your thumb.

SAFETY LABEL

You can mark the pipette application your initials the
calibration date, etc.on the safety label.
Remove the clear plastic window on the edge closest to
the push button (use the service tool that comes with the
pipette, or a screwdriver). Mark the adhesive label with a
felt-tipped or other pen and snap the window back in place.

PIPETTING TECHNIQUES

Push and release the push button slowly at all times
particularly when working with high viscosity liquids. Never
allow the push button to snap back.
Make sure that the tip is firmly attached to the tip cone.
Check for foreign particles in the tip.
Before you begin your actual pipetting work, fill and empty
the tip 2-3 times with the solution that you will be pipetting.
Hold the pipette in an upright position while aspirating liquid. The grippy should rest on your
index finger. Make sure that the tips, pipette and solution are at the same temperature.
FORWARD TECHNIQUE
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm and slowly
release the push button. Withdraw the tip from the
liquid touching it against the edge of the reservoir
to remove excess liquid.
3. Deliver the liquid by gently depressing the push
button to the first stop. After a delay of about one
second, continue to depress the push button all
the way to the second stop. This action will empty
the tip.
4. Release the push button to the ready position.If necessary, change the tip and continue pipetting.
REVERSE TECHNIQUE
The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency
to foam easily. The technique is also recommended for dispensing very small volumes. Fill a
clean reagent reservoir with the liquid to be dispensed.

PIPETTE OPERATION

6
Figures:
A = Ready position
B = First stop
C = Second stop
A 1 2 3 4
B
C