Preparing The Osmometer K-7400; Calibration; Pre- Calibration - Knauer K-7400 Benutzerhandbuch

Using the Osmometer K-7400

Preparing the Osmometer K-7400

SOP 1

Calibration

Pre- calibration

SOP 2
The instrument should be switched ON at least 5 minutes before
use.
The vibrator key may be pressed to check the stirrer. It will run for a
second.
Optionally a printer can be connected to the RS232 socket. The result of
each measurement will be printed automatically in the format:
Measured 0400 mOsmol / kg
23.04.15 08:24:35
After a calibration run you get the outprint:
Calibrated 1234 mOsmol / kg
23.04.15 07:36:17
In case of interrupted runs (caused by any error) the screen displays the
corresponding error message and you get the printout
(NO) FREEZE
23.04.15 09:06:04
without specifying the individual error.
Preparing a Measurement
1. Place 0.15 ml sample or calibration solution into a clean, dry
measurement vial.
2. Put the vial all the way into the adapter. The meniscus of the liquid
must be horizontal.
3. Place the measuring head on the instrument in such a way that the
vial extends into the cooling cavity.
4. Thus the instrument is ready for calibrating or measuring.
If you intend to use both types of measuring heads alternatively with the
osmometer, it is necessary after each exchange to adapt the instrument
to the new measuring head by a pre-calibration procedure as described
in SOP 2 on page 13.
Pre- calibration after measuring head exchange
1. Prepare the instrument according SOP 1 for a measurement of
deionised water.
2. Select the CALIBRATE-T screen.
3. Press the start key. While the pre-calibration is running the screen
displays the sample temperature and the elapsed time.
0000 mOsmol > +5.43°C
01:23
At any time the run can be interrupted pressing the STOP key.
4. After finishing the run on the screen is displayed „successful". The
previous measuring calibration will be aborted.
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