Summary Of Darkfield Observation Procedures - Optika Italy B-380 Serie Handbuch

9. Summary of darkfield observation procedures
Set the main swith to "ON" and adjust light intensity
Place a slide on the stage
Insert 10x objective into the light path
Focus the specimen
Lower the condenser, place a drop of oil on the condenser
front lens and raise the condenser to its original position
Swing nosepiece into an intermediate position between 40X
and 100X and put a drop of oil on the slide
Insert 100X into the light path and focus the specimen
Rotate iris diaphragm of 100X objective to obtain darkfield
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
effect
Adjust brightness
Begin observation
(Used commands)
Main switch /
brightness adjustment knob
Slide holder
X/Y movement knob
Nosepiece
Coarse and fine focus knobs
Observation head
Diopter adjustment ring
Condenser centering
Condenser height adjustment knob
Nosepiece
Nosepiece
Coarse and fine focus knobs
Main switch /
brightness adjustment knob
100x OIL/IRIS Objective
Main switch /
brightness adjustment knob
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