Recalibration - Optipette OP2 Bedienungsanleitung

• When pipetting acids or corrosive solutions which emit
vapours, it is recommended to disassemble the shaft
and to rinse the piston and seal with distilled water after
finishing the pipetting operation.
The pipettes are UV resistant which was confirmed by
our tests. The recommended distance from the radiation
source to exposed element should be not less than
50 cm. Prolonged and very intense UV exposure
can cause de-coloration of pipette parts, without affect-
ing its performance.

8 - RECALIBRATION

HTL pipettes are calibrated by gravimetric me thod, using
HTL tips and distilled water, at the temperature 20±1°C,
according to EN ISO 8655 standard.
If during pipette operation you find that the accuracy
error (the difference between the real aspirated volume
and the preset volume) exceeds the permissible value
given in the table in section 1, the pipette recalibration
procedure should be carried out.
Before starting the recalibration it is necessary to check
whether the following requirements have been fulfilled
during error determination:
• the ambient temperature, and the temperature of the
pipette, tips and water was identical
• the density of the liquid used is close to that of distilled
water
• the balance with appropriate sensitivity has been used.
Volume checked [μl]
0.1 - 10
10 - 100
> 100
• mg/μl conversion factor has been taken into ac count
• the requirements given in sections 3 and 7 have been
fulfilled
If the above conditions are satisfied and the ac cu racy error
for selected volume given in section 1 exceeds the per-
missible value, the pipette recalibration procedure
should be carried out.
The recalibration can be performed within one full turn
of the key to the right or to the left only.
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Balance sensitivity [mg]
≤ 0.001
≤ 0.01
≤ 0.1
Recalibration conditions:
• Ambient temperature and the temperature of the
pipette, tips and liquid should be within the range 20-
25°C and stabilised during weighing within ±0.5°C
• Measurements should be conducted using distilled
water
• Balance sensitivity should be suitable for the vo lume to
be controlled
Recalibration procedure:
• Set the dose volume depending on the pipette volume
according to the following table:
Range Preset
Model of the pipette volume
volumes [μl] [μl]
OP2 0.1 - 2 0.2
OP10 0.5 - 10 0.5
OP20 2 - 20 2
OP50 5 - 50 5
OP100 10 - 100 10
OP200 20 - 200 20
OP250 50 - 250 50
OP1000 100 - 1000 100
OP5000 500 - 5000 500
OP10000 1000 - 10000 1000
• Perform 5 aspirations, weigh each one and calculate
the average value of the aspirations.
• Calculate average aspirated volume in [μl] multiplying
the average aspiration amount [mg] by the distilled
water density coefficient [μl/mg], which depends on
temperature and pressure according to the following
table:
Temperature [°C]
20
21
22
23
24
25
ENGLISH
Volume change
ΔV for full turn
Permissible
of the
volumes
calibration key
[μl]
[μl]
(24 increments)
0.176 - 0.224 0.06
0.48 - 0.52 0.33
1.94 - 2.06 0.63
4.875 - 5.125 2.50
9.84 - 10.16 2.50
19.76 - 20.24 6.30
49.5 - 50.5 6.30
98.4 - 101.6 25.00
494 - 506 125.00
975 - 1025 250.00
Pressure [kPa]
95.0 101.3 105.0
1.0028 1.0029 1.0029
1.0030 1.0031 1.0031
1.0032 1.0033 1.0033
1.0034 1.0035 1.0036
1.0037 1.0038 1.0038
1.0039 1.0040 1.0040
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