Setting The Volume - Optipette OP2 Bedienungsanleitung

- in 2 and 10 μl pipettes (Fig. 6D)
The 2 μl - 10 μl pipettes may require the use of an ejector
cap "M" to efficiently eject certain brands of pipette tips.
Simply place the cap on the bottom of the pipette shaft
and slide the cap upwards until it surrounds the bottom
of the tip ejector.
The OPTIPETTE is a high quality instrument which offers
excellent accuracy and precision.
The accuracy and precision (repeatability) of liquid vol-
ume depend on the quality of tips used. The values for
accuracy and precision given in the table below were
obtained using HTL tips. Only those tips guarantee cor-
rect operation of the pipettes and ensure accuracy and
precision of liquid sampling.
Volume
Model Cat. no.
[μl]
0.2
OP2 5601 1.0
Max 2.0
Min 0.5
OP10 5602 5.0
Max 10.0
Min
OP20 5603 10
Max 20
Min
OP50 5607 25
Max 50
Min 10
OP100 5604 50
Max 100
Min 20
OP200 5605 100
Max 200
Min 50
OP250 5600 125
Max 250
Min 100
OP1000 5606 500
Max 1000
Min 500
OP5000 5608 2500
Max 5000
Min 1000
OP10000 5609 5000
Max 10000
The accuracy and precision are obtained with HTL tips,
using a gravimetric method, performing at least 10 mea-
3
Accuracy Precision Tip
[%] [%] μl
± 12.0 ± 6.0
± 2.7 ± 1.3
± 1.5 ± 0.7
10 μl
± 4.0 ± 2.8
± 1.0 ± 0.6
± 0.5 ± 0.4
2 ± 3.0 ± 1.5
± 1.0 ± 0.5
± 0.8 ± 0.3
5 ± 2.5 ± 2.0
± 1.0 ± 0.6
± 0.8 ± 0.4
200 μl
± 1.6 ± 0.80
± 0.8 ± 0.24
± 0.8 ± 0.20
± 1.2 ± 0.60
± 0.8 ± 0.25
± 0.6 ± 0.20
± 1.0 ± 0.4
± 0.8 ± 0.3 300 μl
± 0.6 ± 0.3
± 1.6 ± 0.40
± 0.7 ± 0.20 1000 μl
± 0.6 ± 0.15
± 1.2 ± 0.50
5000 μl
± 0.6 ± 0.20
± 0.5 ± 0.15
± 2.5 ± 0.6
10000 μl
± 0.8 ± 0.3
± 0.5 ± 0.2
surements of distilled water at a temperature of 20±1°C,
according to EN ISO 8655 standard.
The pipette design enables the user to perform the
recali bration process according to the rules presented in
section 8.

2 - SETTING THE VOLUME

The counter displays three figures to be read from top to
bottom. Additional to the figures on the lower wheel are
printed graduations to enable a volume setting in the
range increment of each pipette model.
Examples of the meaning of the black and red figures:
Pipettes OP2
Red figures at the bottom = 1/100 μl
Increment = 0.002 μl
Pipettes OP10, OP20,
Red figures at the bottom = 1/10 μl
Increment = 0.02 μl
Pipettes OP50, OP100,
OP200, OP250
Black figures only = 1 μl
Increment = 0.2 μl
Pipettes OP1000, OP5000,
Red figures at the top = ml
Increment =
Pipettes OP10000
Red figures at the top = ml
Increment = 20 μl
The volume of the pipette is set by the black adjustment
knob (Fig. 1B). To attain the maximum accuracy, set vol-
ume must be approached from a higher value by dimi -
nishing counter readings.
ENGLISH
OP2
OP10 OP20
OP50, OP200,
OP100 OP250
OP1000 OP5000
2 μl 10 μl
OP10000
4