Adjustment - Thermo Scientific Finnpipette Novus Bedienungsanleitung

Adjustment

Two point calibration
Normally the two-point calibration should be used.
1. Do the pipetting series with both max. and min. volumes.
2. Calculate the actual results.
3. Press MENU and select options with SCROLLKEY and accept with OK.
4. Select CALIBRATE and press OK.
5. Select two points and confirm with OK. The target max. and target min. volumes are
shown on the display.
6. Press EDIT and change the actual max. volume with the SCROLLKEY and accept with OK.
7. Change the actual min. volume with the SCROLLKEY and accept with OK.
8. A text SAVE? appears on the display.
9. Accept with YES
10. The adjustment has been changed.
One point calibration
The one point calibration can be used if a single specific volume must be calibrated. The calibration
volume can be selected from the entire volume range. Note that the inaccuracy of other volumes
changes also and the performance for other volumes cannot be assured.
1. Do the pipetting series with calibration volume.
2. Calculate the results.
3. Press MENU and select options with SCROLLKEY and accept with OK.
4. Select CALIBRATE and press OK.
5. Select one point and confirm with OK. The calibration volume is shown on the display.
6. Press EDIT and change the calibration volume with the SCROLLKEY and accept with OK.
7. Change the actual volume with the SCROLLKEY and accept with OK.
8. A text SAVE? appears on the display.
9. Accept with YES
10. The adjustment has been changed.
Formulas for calculating results
Conversion of mass to volume
V = (w + e) x Z V = volume (µl)
w = weight (mg)
e = evaporation loss (mg)
Z = conversion factor for µl/mg conversion
Evaporation loss can be significant with low volumes. To determine mass loss, dispense water to
the weighing vessel, note the reading and start a stopwatch. See how much the reading decreases
during 30 seconds (e.g. 6mg = 0.2mg/s).
Compare this to the pipetting time from tareing to reading. Typically pipetting time might be 10
seconds and the mass loss is 2 mg (10s x 0.2mg/s) in this example. If an evaporation trap or lid on
the vessel is used the correction of evaporation is usually unnecessary.
The factor Z is for converting the weight of the water to volume at test temperature and pressure.
A typical value is 1.0032µl/mg at 22°C and 95 kPa. See the conversion table on page 85.
Inaccuracy (systematic error)
Inaccuracy is the difference between the dispensed volume and the selected volume of a pipette.
A = V - V A = inaccuracy
0
V = mean volume
V = nominal volume
0
Inaccuracy can be expressed as a relative value:
A% = 100% x A / V
0
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