Peqlab peqSTAR series Kurzanleitung Seite 7

Short Manual peqSTAR thermal cyclers
Available commands:
Lock Lid
Locks the lid avoid accidental opening.
Unlock Lid
Unlocks the lid in case that the lid has been
locked in a previous program step.
Heat Lid
Turns the lid heating on/off.
Temperature
Temperature step for definable time,
Temperature and Time Incr./Decr. function.
Gradient
Realizes different temperatures at the same
time for protocol optimization
(see 'FlexGradient' below for more
information).
Sample program
Create a new program with 'New'. Enter a program name and confirm with OK
functions.
01
Heat Lid
To enter another program step select the desired command out of the list of the available command and add it to
the program window with the '+' arrow. Thereupon the corresponding parameters will be asked.
02
Temp
03
-
07
Cycle
04
Temp
PEQLAB_v1212E
Heated Lid on, temperature 110 °C
A 'Heat Lid' step is inserted in each program by default as first step. This step can be edited
or deleted later. The standard settings could be changed under 'System' => 'Firmware' =>
'Settings'.
For 'Store' or 'Temp' steps < 30 °C the lid heating is automatically switched off.
Denaturation at 95 °C for 2 minutes
Open the 'Cycle' function
→ Enter number of cycles – 30 cycles
→ 3 x 'Temp' step
→ Terminate 'Cycle' function with 'Close Cycle'
Denaturation at 95 °C for 30 seconds
6
Cycle
Combines Open Loop, Close Loop,
Temperature, Ramp and Gradient.
Pause
Interrupts the program temporarily at the
definable temperature.
Store
Holds the definable temperature, turns off
the heated lid automatically.
Start Cycle
Manual loop beginning.
Close Cycle
Manual loop ending.
OK to access the programming OK OK