Recalibration - Optipette OP8-10 Bedienungsanleitung

• check if the tips are fitted properly,
• during operation, the pipette must be held vertically,
tips should be immersed in liquid to the depth of 2 to 4
millimeters and the pipetting button should be
depressed and rele ased slowly and evenly,
• new tips must be pre-wetted prior to pipetting, by draw-
ing and expelling the liquid to be me asured. This is
especially important when wor king with liquids of visco-
sities and densities different from that of water and
in temperatu res different from ambient temperature,
• when pipetting liquids which tend to wet walls of tips,
such as serums, proteins or organic solvents, measuring
must be performed much slower than with other liquids,
• tips must be replaced with new ones, when chan ging
from one liquid to another, or if drops of liquid remain
inside the tips.

5 - RECALIBRATION

HTL pipettes are calibrated by gravimetric met hod, using
HTL tips and distilled water, at the temperature 20±1°C,
according to EN ISO 8655 standard.
If during pipette operation you find that the accuracy
error (the difference between the real aspirated volume
and the preset volume) exceeds the permissible value
given in the table in section 1, the pipette recalibration
procedure should be carried out.
Before starting the recalibration it is necessary to check
whether the following requirements have been fulfilled
during error determination:
• the ambient temperature, and the temperature of the
pipette, tips and water was identical
• the density of the liquid used is close to that of distilled
water
• a balance with appropriate sensitivity has been used
Volume checked [μl]
0.1 - 10
10 - 100
> 100
• mg/μl conversion factor has been taken into ac count
• the requirements given in sections 3 and 4 have been
fulfilled
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Balance sensitivity [mg]
≤ 0.001
≤ 0.01
≤ 0.1
If the above conditions are satisfied and the ac cu racy
error for selected volume given in section 1 exceeds the
permissible value, the pipette recalibration procedure
should be carried out.
The recalibration can be performed within one full turn
of the key to the right or to the left only.
Recalibration conditions:
• Ambient temperature and the temperature of the pi -
pette, tips and liquid should be within the range 20-25°C
and stabilised during weighing within ±0.5°C
• Measurements should be conducted using distilled
water
• Balance sensitivity should be suitable for the vo lume to
be controlled
Recalibration procedure:
• Set the dose volume depending on the pipette volume
according to the following table:
Range
Preset
of the pipette
Model volume
volumes
[μl]
[μl]
OP8-10
0.5 - 10 0.5
OP12-10
OP8-50
5 - 50 5
OP12-50
OP8-200
20 - 200 20
OP12-200
OP8-300
50 - 300 50
OP12-300
• Perform three aspiration series (each series should
include the aspirations from all channels), weigh each
time and calculate the average value of the aspirations.
• Calculate average aspirated volume in [μl] multiplying
the average aspiration amount [mg] by the distilled
water density coefficient [μl/mg], which depends on
temperature and pressure according to the following
table:
ENGLISH
Volume
change DV
Permissible
for full turn of the
volumes
calibration key
[μl]
[μl]
(24 increments)
0.45 - 0.55 0.33
4.8 - 5.2 1.67
19.4 - 20.6 6.30
49.2 - 50.8 10.00
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