Inhaltsverzeichnis
Werbung
Verfügbare Sprachen
Verfügbare Sprachen
Short protocol Homogeniser Precellys
Which lysis kit matches the starting material?
Ceramic Kit 5.0/2.8 mm, 7 ml (CK50L)
Ceramic Kit 2.8 mm, 7 ml (CK28L)*
Keramik-Kit 1.4 mm, 7 ml (CK14L)
Glass/Ceramic Kit, 7 ml (SKL)*
Ceramic Kit 0.1 mm, 7 ml (CK01L)
Glass Kit 0.1/0.5 mm, 7 ml (VKML)
*
if needed, grinding efficiency can be improved by adding single ceramic beads 5.0 mm or 6.8 mm (CK50P or CK68P) manually
Steel Kit 2.8 mm, 2 ml (MK28, MK28R*)
Ceramic Kit 6.8 mm, 2 ml (CK68R*)
Ceramic Kit 5.0/2.8 mm, 2 ml (CK50R*)
Ceramic Kit 2.8 mm, 2 ml (CK28, CK28R*)
Ceramic Kit 1.4/2.8 mm, 2 ml (CKM)
Ceramic Kit 1.4 mm, 2 ml (CK14)
Glass/Ceramic Kit, 2 ml (SK38)
Glass Kit 0.5 mm, 2 ml (VK05)
Glass Kit 0.1 mm, 2 ml (VK01)
Ceramic Kit 1.4 mm, 0.5 ml (CK14S)
Glass Kit 0.5 mm, 0.5 ml (VK05S)
Further user tips
To preserve the integrity of the target molecule homogenisation should never be more powerful than
required (see Fig. A). For example, excessive grinding can cause shearing of genomic DNA (see Fig. B).
Frictional heat occurring during homogenising generally has no negative effect on nucleic acids. How-
ever, any heat should be avoided if native proteins will be analysed. For this purpose the optional use of
the Cryolys Cooling Module for the Precellys
lysis buffers grinding energy must be increased, because the beads are slowed down by the foam. The
reagent peqGOLD TriFast
While its ingredients (amongst others phenol and GTC) effectively support cell lysis, it is almost non-
foaming. For starting material like soil samples, bacteria, yeast, fungi, animal and plant tissue optimised
kit combinations are available for the homogenisation and downstream nucleic acid isolation using sil-
ica-based centrifugation columns (see 'TECHNICAL SUPPORT and ORDERING INFORMATION').
Fig. A)
PEQLAB_v0314_E
®
24 / 24-Dual
human & animal tissue
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
*
MK28R & CK28R tubes are mechanically reinforced, however, prone to stronger embrittlement in liquid nitrogen
x
x
x
x
®
24/Precellys
™
has a very high lysis potential for the isolation of RNA, DNA or proteins.
plant tissue
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
0.5 ml tubes are ideally suited for small sample volumes < 100 μl or < 10 mg
®
24-Dual is recommended. If using foaming
27
microorganisms/cells
x
x
x
x
x
x
x
x*
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
Fig. B) Mechanical stability of DNA.
Genomic
DNA
from
Arabidopsis
leaves
was
isolated
using
peqGOLD Plant DNA Mini Kit (ho-
®
mogenised in the Precellys
24). After-
wards aliquots of the same amount
were
exposed
increasing
energies in CK14 tubes using the
homogeniser Precellys
®
24 and sepa-
rated on an agarose gel ('M' = peq-
GOLD 1 kb DNA-ladder).
x
.
x
x
x
.
x
.
the
grinding
Quicklinks ausblenden:
Werbung
Kapitel
Inhaltsverzeichnis
Fehlerbehebung
