Sartorius Minisart RC Schnellstartanleitung Seite 2

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Minisart -RC | SRP | NY | PES
Sartorius Syringe Filters: Reliable and Ultrapure
Sartorius ultrapure Minisart
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Minisart filters are manufactured at our own plant in Goettingen, Germany, and are required
to meet the strictest quality standards. As a result, Sartorius sets the standards in both high flow
rates and total throughput. At the same time, these syringe filters are virtually free of extract-
ables, delivering an ultrapure filtrate.
Choose the right Minisart
UHPLC, LC/MS or a different analytical method. All Minisart
are certified for HPLC. Our recommended syringe filters for LC/MS and other analytical proce-
dures are identified accordingly.
Applications
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– Minisart RC for ultracleaning of aqueous and organic solutions
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– Minisart NY for ultracleaning of aqueous and organic solutions
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– Minisart PES specially designed for ultracleaning of aqueous protein solutions
(containing up to 30% MeOH)
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– Minisart SRP with a hydrophobic PTFE membrane for ultracleaning of aggressive liquids
and gases
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– Minisart RC|SRP|NY, individually sterile-packaged (identified by suffix –ACK), for immediate
sterile filtration of liquids
Instructions for Use
a)
If possible, draw a slight amount of air (at least 1 ml) into a syringe (a). Then fill the syringe with
the liquid to be filtered: pull the plunger upwards to draw this liquid from a suitable container
into the syringe (b). If required, remove any remaining liquid from the tip of the syringe and
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attach a Minisart to the luer lock or luer slip connector on the filled syringe (c). Apply consistent
pressure to press in the plunger of the syringe in order to filter the liquid through the Minisart
filter into a suitable vial for collection (d). Afterwards, press the plunger all the way in so that the
air cushion initially created will discharge any liquid remaining in the inlet and outlet of the
filter. As a result, this will reduce the hold-up volume.
Caution!
1. Take care when using syringes with a volume of less than 10 ml, as they can easily generate a
pressure greater than the maximum recommended pressure resistance of 6 bar (87 psi) for
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Minisart . Therefore, slowly press in the plunger on a syringe with a volume of less than 10 ml.
As soon as you detect considerable resistance (= max. filter pressure capacity), do not use force
while continuing to press the plunger. Otherwise, you can damage the filter, and filtration will
no longer guarantee an ultrapure filtrate.
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2. Minisart is designed for bidirectional use. However, once you have selected one direction
of filtration, be sure to maintain this direction. Never use the same syringe filter for both
directions! For sterile filtration according to the BCT (see Table 1), you MUST use Minisart
in the following direction only: from the female luer inlet to which the syringe is attached to
the male luer slip outlet.
Note
1. If you would like to achieve maximum recovery of your sample to be filtered, detach the
syringe from the syringe filter and draw air once more into the syringe. After reconnecting
the syringe and filter, use the air cushion created in this way to press out the remaining liquid.
To do so, you will have to apply pressure beyond the bubble point of the particular Minisart
membrane incorporated (see Table 1). This will reduce the hold-up volume to the lowest
possible amount.
2. Our filters are leading in terms of purity (see also recommendation for various analytical
procedures), so prerinsing is usually not necessary. For extremely sensitive analytical methods,
you may need to additionally prerinse the particular syringe filter you use with 0.5–2 ml of
ultrapure water or ultrapure running buffer or wash buffer. This way, you can be sure that you
have removed even the slightest traces of extractables. To avoid diluting exceptionally small
sample volumes as far as possible, apply the technique described (step 1 above) to press out
the remaining traces of rinse liquid using the air cushion before performing actual filtration.
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3. Use a Minisart for only one sample to reliably prevent carrying over residues of one sample
to the next.
4. You will find an overview of chemical compatibilities in the 'Minisart
Guide' on our website.
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filters are ideal for preparation of analytical samples.
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syringe filter to optimize preparation of your samples for HPLC,
b)
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syringe filters listed below
c) d)
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Chemical Compatibility
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RC
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