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OPERATION
The Tubes
The Hybridisation Tubes must be checked regularly and replaced if any damage
occurs. Any damage to the ends of the tubes can cause them to break when the end
caps are inserted.
Each tube must be fitted with a radiation shielding end cap if you are using a
radioactive probe. These end caps are supplied with all tubes which are supplied with
the HB-1D and any other tubes supplied after December 1999. Normal handling
procedures must be observed when handling any tube.
Load the membrane and reagents according to which size hybridisation tube you
are using.
Large tube (80mm diameter) assembly
Remove the tube end cap which has the holes in it. Place the filters in the tube
and replace the end cap. A small quantity of Techne O-ring grease, see the list of
accessories, must be smeared on the seal to facilitate the insertion of the end cap.
Remove the spindle cap and the liquids appropriate to the protocol may then be added
through the hole in the spindle using a syringe. Replace the spindle cap and press it
onto the spindle so that it clicks into place. Note: the tube will not rotate properly if the
cap is not fitted securely
Spindle Cap
End Cap
Sealing
Fill & Drain
Ring
Mini tube (4 x 32mm dia) assembly
Unscrew the end cap of the tube. Place the filters and the liquids appropriate to
the protocol in the tube and replace the end cap. Fix the tube into a multi-tube holder,
using the springs, and ensure that there are four tubes in the holder. The end caps
must be as shown, on alternate ends. The multi-tube holder will not rotate properly if
there are not four mini tubes in it.
End Cap
Glass Tube
Spring
Tubes
H B - 1 D O P E R A T O R ' S M A N U A L
End Cap Blank
Cap
15
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