Step 3
Step 4
Flushing
After the two pins of the male connector are "clicked" into place, join the grippers
at the bottom of each membrane tape together. The grippers should also "click" into
place. Remove the membrane horizontally.
(A) Slide the collar that has been retracted back in place and (B) turn it clockwise until
the lock "clicks" into place.
(A)
(B)
Once the cassette and the assembly is connected we recommend to flush
the loop.
– Fill the recirculation bag with 5l of sterile water (WFI).
Retentate and Permeate valves are fully open.
Set delta P to a value of 1 bar.
Flushing the system to the drain.
Refill the recirculation bag with 10l of sterile water (WFI) and pause for 15 min.
Set delta P to a value of 1 bar.
– Restrict the retentate to an extent that a ratio between the Permeate flux to
Retentate of 1:1:
Discard the permeate and retentate
Pins
Click
Grippers
"Click"
Installation and Starting Up
9