Measuring Range - Hellma Analytics TRAYCELL 2.0 Schnellstartanleitung

MEASURING RANGE

 By employing a variety of caps (path lengths), and thus virtual dilution factors,
the necessity to dilute samples is significantly reduced, which allows the
concentration range to be significantly increased as a result.
Example of use: Nucleic acid quantitation
 A solution's ratio of absorbance at 260 nm (A260) is used to determine its
nucleic acid content. The following calculation de-
rived from the Beer-Lambert law is used here:
Concentration [ng/μl] = absorbance (260 nm) x sample-specific factor x virtual dilution factor
Example of use: Protein quantitation
 A solution's absorbance at 280 nm (A280) is often used to determine its protein
content. Alternatively, proteins can also be measured through absorbance
at 230 nm or in colorimetric assays. The following calculation derived from
the Beer-Lambert law is used to perform direct measurements at 280 nm:
Concentration [mg/ml] =
1
sample specific factor
On our website you can download a helpful Excel
tool,with which you can calculate the concentrations
of nucleic acids and proteins:
www.hellma.com/traycell-en
× absorbance (280 nm) × virtual dilution factor