Autoclaving; Calibration; Checking Calibration - ahn pipet4u Bedienungsanleitung

Instruction manual
It is recommended to send the multi channel pipettes to
your supplier or manufacturer for cleaning and greasing.
Autoclaving - The
autoclavable by steam at 121°C, 1.05 bar for 15 min.
Autoclave the complete pipette without any
disassembling. After autoclaving under the above
mentioned conditions allow the pipette to cool and dry for
6 hours before use. It is not required to re-calibrate the
pipette after autoclaving. If the pipette is autoclaved
frequently, the piston and springs should be greased
with the lubricant supplied with each pipette to maintain
smooth movement. See section „Disassembling/
reassembling and cleaning".
Calibration - Each pipette calibration has been factory
checked and certified according to EN ISO 8655. It is
recommended to check the calibration at least once a
year, for regularly used pipette.
Checking calibration - Performance testing should
take place in a draught-free room with stable
environment with relative humidity above 50 % and
constant (
°C. The weighing vessel, pipette, tips and destilled water
shall have stood in the room for a sufficient time, at least
2 hours, to reach equilibrium with the room conditions.
Use an analytical balance with a readability of 0.01 mgs.
10 measurements for each test volume shall be carried
out. The test volume for fixed volume pipettes is the
nominal volume (greatest volume selectable by the user
and specified by the manufacturer) and for variable
volume pipettes at least three volumes shall be tested
(nominal volume, 50 % of nominal volume, 10 % of
nominal volume). For checking calibration of multi
channel pipette, each channel should be considered as
an individual pipette.
Procedure
1. Select the test volume. Do not change the setting
during the test cycle of 10 measurement.
2. Fit the selected tip(s) to the pipette cone(s).
3. Fill the tip(s) with destilled water and condition the
pipette before testing by aspirating and dispensing
the destilled water in the tip(s) five times. Afterwards
discard the tip(s).
4. Attach a new tip(s) on the pipette cone(s) of pipette
and pre-wet the tip(s) once.
5. Aspirate the destilled water and pipette it into the
weighing vessel (use forward pipetting technique,
page 8).
6. Weigh the pipetted quantity with an analytical
balance and record the weight. Repeat the test
cycle until 10 measurements have been recorded.
7. Convert the recorded masses to volumes, calculate
the mean volume, systematic error and random
error as described in EN ISO 8655-6:2002, part 8
8. Compare the systematic error (inaccuracy) and the
random error (imprecision) with the values of
specification table, page 16.
10
pipet4u pro
0,5 °C) temperature between 15 °C and 30
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pipettes are fully