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Start up of cassettes
After 30 minutes, sterilization is completed. Close valves V
t
Close valve V
on the feed line, V
t
1
filtered stabilizing pressure of 14.5 psi|1.0 bar|100 kPa. At this point, shut off the
steam supply.
Allow the holding device to cool down for 90 minutes. During cool down V
t
remain open.
After 90 minutes cool down time close V
t
Vent system through upper permeate valve V
t
vent excess pressure. If sterile air venting is required, please implement
a sterilizing air filter.
Using a torque wrench, alternately retighten the stainless steel pressure compen-
t
sating nuts to the torque indicated [Æ Chapter "5.2 Clamping Forces", page 12].
After steam sterilization is completed flush filter cassettes with RO water until
t
system has reached room temperature, this will also ensure that the membranes
will not dry out.
5.6.2
In-Line Steam Sterilization of Sartocon
Ultrafilter cassettes are sterilized through the retentate and the permeate flowpaths
simultaneously to maintain equal pressure within the cassette.
V
2
Steam
generator
Retentate Line
V
5
Stabilizing pressure inlet
P
V
1
Feed Line
Diagram of the crossflow system for in-line steam sterilization
of polyethersulfone Ultrafilter cassettes.
V
–V
: valves; P
–P
: pressure gauge, P: pump
1
6
1
4
Assemble system as shown on the diagramm.
t
Open V
, V
, V
, V
and V
, close V
t
2
3
4
6
1
Install cassettes into filter holder.
t
Attach a RO water source to the feed line.
t
Rinse cassette with RO water for 5 minutes at an inlet pressure of 29 psi|2 bar|
t
200 kPa and retentate pressure of 7 psi|0.5 bar|50 kPa. Adjust valve settings to
achieve pressure values if needed.
Drain the system.
t
Close valve V
. Connect V
t
2
2
Exchange torque the normal operation nuts to the pressure compensating torque
t
nuts. For specific information related to the filter holder see user manual of the
installed filter holder.
Use a torque wrench to tighten the compensation nut to the first notch
t
on the retentate line. Open V
2
.
5
. Do not open V
3
Ultrafilter Cassettes
®
P
2
V
6
P
P
1
3
Cassette
V
3
Permeate
P
4
V
4
Permeate
.
5
at the retentate line with a steam generator.
|V
.
3
4
to apply sterile
5
has to
5
completely. Only
3
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