Performance Characteristics; Symbol Legend - Remel Rapid Ana Ii System Kurzanleitung

Table 3. Quality Control Chart for RapID ANA II Panels
Organism URE BLTS
Clostridium sordellii
a
+ –
ATCC® 9714
Parabacteroides distasonisa
– +
ATCC® 8503
Bacteroides uniformis
– +
ATCC® 8492
+, positive; –, negative; V, variable
Key indicator strains demonstrate acceptable performance of the most labile substrate in the system and reactivity in a significant number of wells, according to Clinical and Laboratory Standards Institute
a
recommendations for streamlined quality control.
36
• Formulations, additives, and ingredients of culture media
vary from manufacturer to manufacturer and may vary from
batch to batch. As a result, culture media may influence
constitutive enzymatic activity of designated quality
control strains. If quality control strain results differ from
the patterns indicated, a subculture onto medium from a
different batch or from another manufacturer will often
resolve quality control discrepancies.
15. LIMITATIONS
1. The use of RapID ANA II System and the interpretation
of results requires the knowledge of a competent
microbiologist, familiar with laboratory procedures, who
is trained in general microbiological methods and who
judiciously makes use of training, experience, specimen
information, and other pertinent procedures before
reporting the identification obtained using RapID ANA II
System.
2. Specimen source, aerotolerance, Gram stain characteristics,
and growth on selective agars should be considered when
using RapID ANA II System.
3. RapID ANA II System must be used with pure cultures of
test organisms. The use of mixed microbial populations or
direct testing of clinical material without culture will result
in aberrant results.
4. RapID ANA II System is designed for use with the taxa listed
in the RapID ANA II Differential Charts. The use of organisms
not specifically listed may lead to misidentifications.
5. Expected values listed for RapID ANA II System tests may
differ from conventional test results or previously reported
information.
6. The accuracy of RapID ANA II System is based upon the
statistical use of a multiplicity of specially designed tests and
an exclusive, proprietary database. The use of any single test
found in RapID ANA II System to establish the identification
of a test isolate is subject to the error inherent in that test
alone.
Table 4 - RapID ANA II Differential Chart: Cocci
Organism
URE BLTS αARA ONPG αGLU βGLU αGAL αFUC NAG
Anaerococcus hydrogenalis 0 0 0 0
a
Anaerococcus prevotii 2 0 0 0
b
Anaerococcus tetradius 88 5 0 12
c
Blautia producta d 0 51 0 99
Finegoldia magna 0 0 0 0
e
Gemella morbillorum 0 0 0 18
f
Parvimonas micrag 0 0 0 0
g
Peptoniphilus asaccharolyticu 0 0 0 0
s h
Peptoniphilus indolicus 0 2 0 0
i
Peptostreptococcus anaerobius 0 0 0 0
Staphylococcus saccharolyticus 21 0 0 0
Streptococcus constellatus 0 0 0 0
Streptococcus intermedius 0 29 9 99
Veillonella spp. 0 0 0 0
j
a Previously designated as Peptostreptococcus hydrogenalis
b Previously designated as Peptostreptococcus prevotii.
c Previously designated as Peptostreptococcus tetradius.
d Previously designated as Peptostreptococcus productus.
e Previously designated as Peptostreptococcus magnus
f Previously designated as Streptococcus morbillorum.
Table 5 - RapID ANA II Differential Chart: Gram-Negative Bacilli
Organism
URE BLTS αARA ONPG αGLU βGLU αGAL αFUC NAG
Bacteroides caccae 0 79 98 99
Bacteroides eggerthii 0 39 77 93
Bacteroides fragilis 0 89 3 94
Bacteroides ovatus 0 95 96 88
Bacteroides stercoris 0 45 9 22
Bacteroides pyogenes 0 9 0 92
a
Bacteroides thetaiotaomicron 0 92 91 96
Bacteroides uniformis 0 93 81 98
Bilophila wadsworthia 91 0 0 0
Campylobacter gracilis 0 0 0 0
b
Campylobacter ureolyticus 99 0 0 0
c
Capnocytophaga spp. 0 42 0 89
Fusobacterium mortiferum 0 12 0 62
Fusobacterium necrophorum 0 0 0 0
Fusobacterium nucleatum 0 0 0 0
Fusobacterium varium 0 4 0 0
Odoribacter splanchnicus d 0 8 4 95
Parabacteroides distasonis 0 90 67 96
e
Parabacteroides merdae 0 20 12 99
f
Phocaeicola vulgatus 0 3 85 95
i
Porphyromonas asaccharolytica 0 2 0 0
Porphyromonas endodontalis 0 0 0 0
Porphyromonas gingivalis 0 2 0 2
Prevotella bivia 0 2 0 97
Prevotella buccae 0 71 31 91
Prevotella buccalis/veroralis 0 0 0 99
Prevotella corporis 0 0 0 0
Prevotella denticola 0 0 0 99
Prevotella disiens 0 2 0 0
Prevotella intermedia 0 0 0 0
Prevotella loescheii 0 0 0 97
Prevotella melaninogenica 0 1 16 98
Prevotella oralis Group 0 92 0 92
Prevotella oris 0 98 99 88
Pseudoflavonifractor capillosus g 0 87 9 95
Tannerella forsythia 0 78 0 95
h
Wolinella spp. 0 0 0 0
Gram-Variable Bacilli:
Clostridium clostridioforme 0 84 90 84
Clostridium ramosum 0 90 0 84
Mobiluncus curtisii 0 26 2 31
Mobiluncus mulieris 0 26 2 31
Tissierella praeacuta 0 0 0 0
Previously designated as Bacteroides tectum.
a
Previously designated as Bacteroides gracilis.
b
Previously designated as Bacteroides ureolyticus.
c
Previously designated as Bacteroides splanchnicus.
d
Previously designated as Bacteroides distasonis.
e
Before Reagent Addition
αARA ONPG αGLU βGLU αGAL αFUC
– – – – – V
V + + + + –
+ + + + + +

16. PERFORMANCE CHARACTERISTICS

The RapID ANA II System performance characteristics have
been established by laboratory testing of reference and stock
cultures.
5,10, 21-35
17. BIBLIOGRAPHY
1. Blazevic, D.J. and G.M. Ederer. 1975. Principles of
Biochemical Tests in Diagnostic Microbiology. John Wiley &
Sons, New York, NY.
2. Dowell, V.R., Jr. and T.M. Hawkins. 1977. Laboratory Methods
in Anaerobic Bacteriology, CDC Laboratory Manual. U.S.
Dept. of H.H.S. CDC, Atlanta, GA.
3. Holdeman. L.V., E.P. Cato, and W.E.C. Moore. 1977. Anaerobe
Laboratory Manual. 4th ed. Virginia Polytechnic Institute
and State University, Blacksburg, VA.
4. Barnes, E.H. and J.F. Morris. 1957. J. Bacteriol. 73:100-104.
5. Dellinger, C.A. and L.V. Moore. 1986. J. Clin. Microbiol.
23:289-293.
6. Guilbault, G.G. 1970. Enzymatic Methods of Analysis. p. 43-
51. Pergamon Press, New York, NY.
7. Kilian, M. and P. Bulow. 1976. Acta. Pathol. Microbiol. Scand.
B. 84:245-251.
8. Tharagonnet, D., P.R. Sisson, C.M. Roxby, H.R. Ingham, and
J.B Selkon. 1977. J. Clin. Pathol. 30:505-509.
9. Bodansky, O. and A.L. Latner. 1975. Advances in Clinical
Chemistry. Vol. 17, p. 53-61. Academic Press, New York, NY.
10. Celig, D.M. and P.C. Schreckenberger. 1991. J. Clin. Microbiol.
29:457-462.
11. Nagatsu, T., M. Hino, H. Fuyamada, T. Hayakawa, S.
Sakakibara, Y. Nakagawa, and T. Takemoto. 1976. Anal.
Biochem. 74:466-476.
12. Norris, J.R. and D.W. Ribbons. 1976. Methods in Microbiology.
Vol. 9, p. 1-14. Academic Press, New York, NY.
13. Peterson, E.H. and E.J. Hsu. 1978. J. Food Sci. 43:1853-1856.
14. Westley, J.R., P.J. Anderson, V.A. Close, B. Halpern, and E.M.
Lederberg. 1967. Appl. Microbiol. 15-822-825.
PO₄ LGY GLY PRO
98 0 0 0 0 2 9 14 2
9 1 0 0 0 9 11 21 11
88 16 5 0 0 0 33 96 0
99 96 56 0 52 0 0 2 0
2 0 0 0 2 6 95 96 2
80 1 0 0 0 68 71 73 88
4 0 0 0 5 93 95 98 92
3 1 0 0 0 4 11 22 5
0 0 0 0 0 99 28 76 0
96 0 0 0 2 0 0 8 92
0 0 0 0 0 18 0 0 0
46 91 18 0 12 94 82 97 6
92 95 8 0 21 92 92 98 78
4 0 0 0 0 59 2 9 1
g Previously designated as Micromonas micros and prior to that
Peptostreptococcus micros.
h Previously designated as Peptostreptococcus asaccharolyticus.
i Previously designated as Peptostreptococcus indolicus.
j Consists of three species from human clinical specimens: V. parvula, V. dispar,
and V. atypica.
PO₄ LGY GLY PRO
88 96 99 97 99 99 96 91 5
99 98 42 0 99 98 97 13 0
98 97 98 98 98 98 98 81 1
98 95 98 93 99 98 96 84 0
99 31 0 70 99 99 98 9 0
99 49 2 93 99 99 99 9 0
98 96 98 98 98 94 98 67 1
99 97 92 93 99 98 98 6 0
0 0 0 0 0 2 0 2 0
0 0 0 0 0 0 0 6 0
0 0 0 0 0 0 16 8 5
96 86 2 1 78 76 99 99 90
2 70 92 0 0 92 28 42 0
0 0 0 0 0 38 0 0 0
0 0 0 0 0 2 0 0 0
0 0 8 0 2 0 16 72 2
0 8 93 92 99 73 91 14 9
99 91 96 0 99 94 96 94 0
38 29 99 0 98 22 98 99 0
99 0 99 99 99 95 98 82 4
0 0 0 94 0 96 92 22 0
0 0 0 0 29 95 98 0 0
0 0 0 0 99 95 96 19 0
99 0 0 91 99 98 98 95 2
99 94 99 0 1 99 97 10 0
99 6 99 8 95 99 99 98 0
98 0 0 29 0 98 96 0 0
99 0 91 99 99 99 99 7 0
99 0 7 0 0 99 98 90 0
99 0 0 93 0 98 98 4 0
98 79 81 80 99 99 99 9 0
92 0 90 92 99 98 99 5 0
96 89 91 78 96 98 98 90 0
99 89 78 98 99 99 98 11 0
30 92 0 98 99 96 90 36 0
99 50 0 99 99 99 98 12 0
0 5 0 0 0 5 0 0 0
85 87 98 0 81 2 93 9 1
99 99 76 0 99 0 29 2 0
99 5 95 15 0 5 28 32 99
99 5 0 15 0 5 28 32 99
0 0 0 0 0 85 88 26 0
Previously designated as Bacteroides merdae.
f
Previously designated as Bacteroides capillosus.
g
Previously designated as Bacteroides forsythus.
h
Previously designated as Bacteroides vulgatus.
i
After RapID ANA II Reagent Addition
NAG PO LGY GLY PRO PAL
4
– – – V + V
+ + + + –
+ + + – – –
15. Fay, G.D. and A.L. Barry. 1974. Appl. Microbiol. 15:822-825.
16. Sutter, V.L. and W.T. Carter. 1972. J. Clin. Pathol. 58-335-339.
17. Holdeman. L.V., E.P. Cato, and W.E.C. Moore. 1987.
Anaerobe Laboratory Manual Update. Supplement to the
4th ed. Virginia Polytechnic Institute and State University,
Blacksburg, VA.
18. Summanen, P., E.J. Baron, D.M. Citron, C.A. Strong, H.M.
Wexler, and S.M. Finegold. 1993. Wadsworth Anaerobic
Bacteriology Manual. 5th ed. Star Publishing Company,
Belmont, CA.
19. Murray, P.R., E.J. Baron, J.H. Jorgensen, M.L. Landry, and
M.A. Pfaller. 2007. Manual of Clinical Microbiology. 9th ed.
ASM Press, Washington, D.C.
20. Forbes, B.A., D.F. Sahm, and A.S. Weissfeld. 2007. Bailey and
Scott's Diagnostic Microbiology. 12th ed. Mosby Elsevier, St.
Louis, MO.
21. Alexander, C.J., D.M. Citron, S. Hunt Gerardo, M.C. Claros,
D. Talan, and E.J. Goldstein. 1997. J. Clin. Microbiol. 35:406-
411.
22. Appelbaum, P.C., C.S. Kaufman, J.C. Keifer, and J.J. Venbrux.
1984. J. Clin. Microbiol. 18:615-621.
23. Appelbaum, P.C., J.W. Depenbusch, and C.S. Kaufman. 1984.
Abstract C-153. Abstracts of the 84th General Meeting of
the American Society for Microbiology. ASM, Washington,
D.C.
24. Burdash, N.M., K.A. Corey, P.J. Fortuna, M.L. Beasely, E.R.
Bannister, and J.P Manos. 1984. Abstract C-150. Abstracts
of the 84th General Meeting of the American Society for
Microbiology. ASM, Washington, D.C.
25. Hamilton, L.T., C. Ayer, and D.N Wright. 1985. Abstract C-159.
Abstracts of the 85th General Meeting of the American
Society for Microbiology. ASM, Washington, D.C.
26. Hansen, S.L. and W.A. Pope. 1984. Abstract C-147. Abstracts
of the 84th General Meeting of the American Society for
Microbiology. ASM, Washington, D.C.
27. Hudspeth, M.K., S. Hunt Gerardo, M.F Maiden, D.M. Citron,
and E.J. Goldstein. 1999. J. Clin. Microbiol. 37:2003-2006.
28. Kaplan, R.L., M.J. O'Brian, and W. Landua. 1985. Abstract
C-163. Abstracts of the 85th General Meeting of the
American Society for Microbiology. ASM, Washington, D.C.
PAL ARG SER PYR IND
29. Karachewski, N.O., E.L. Busch, and C.L. Wells. 1984. Abstract
1 65 2 96 99
C-148. Abstracts of the 84th General Meeting of the
16 96 68 2 0
American Society for Microbiology. ASM, Washington, D.C.
88 97 90 61 0
30. Mangels, J., D. Berkley, and S. Wood. 1984. Abstract C-152.
2 9 6 0 0
Abstracts of the 84th General Meeting of the American
82 98 95 98 0
Society for Microbiology. ASM, Washington, D.C.
93 99 98 4 0
31. Marler, L.M., J.A. Sider, L.C. Wolters, Y. Pettigrew, B.L. Skitt,
88 98 96 86 0
and S.D. Allen. 1991. J. Clin. Microbiol. 29:874-878.
20 96 21 8 99
32. Morgenstern, F. 1984. Abstract C-154. Abstracts of the 84th
81 91 98 0 99
General Meeting of the American Society for Microbiology.
ASM, Washington, D.C.
16 68 18 0 0
0 0 0 0 0
98 99 93 6 0
Table 6 - RapID ANA II Differential Chart: Gram-Positive Bacilli
98 99 96 5 0
Organism
5 38 16 83 0
Actinomyces bovis
Actinomyces israelii
Actinomyces naeslundii
Actinomyces viscosus
Arachnia propionica g
Atopobium minutum
b
Bifidobacterium spp.
Clostridium baratii
Clostridium beijerinckii
PAL ARG SER PYR IND
Clostridium botulinum I
75 98 72 0 0
Clostridium botulinum II
0 32 5 0 99
Clostridium butyricum
79 98 81 74 0
Clostridium cadaveris
0 3 2 0 99
Clostridium clostridioforme
0 2 4 0 98
Clostridioides difficile
p
13 70 22 0 0
Clostridium hastiforme
49 98 77 3 99
Clostridium innocuum
2 3 0 0 99
Clostridium novyi A
0 95 1 0 0
Clostridium paraputrificum
0 93 5 0 0
Clostridium perfringens
2 82 6 3 0
Clostridium ramosum
99 99 95 13 0
Clostridium septicum
60 96 86 91 0
Clostridium sporogenes
0 15 2 0 99
Clostridium subterminale
0 45 0 36 99
Clostridium tertium
41 98 88 99 60
Clostridium tetani
34 99 84 99 99
Collinsella aerofaciens
e
70 98 98 91 0
Cutibacterium acnes
m
14 99 25 2 0
Cutibacterium granulosum
1 85 52 84 0
Eggerthella lenta
f
14 60 45 0 99
Eggerthia catenaformis
0 5 2 0 99
Eubacterium limosum
5 93 27 27 99
Hathewaya histolytica
4 75 24 0 0
Hathewaya limosa
s
5 6 0 0 0
Lactobacillus acidophilus
0 0 0 0 0
Lacticaseibacillus casei
0 98 14 6 0
Limosilactobacillus fermentum
0 0 0 0 0
Lactobacillus jensenii
2 90 29 0 0
Mobiluncus curtisii
0 96 6 0 99
Mobiluncus mulieris
1 4 2 0 0
Paeniclostridium sordellii
0 98 2 0 0
Paraclostridium bifermentans
0 5 2 0 0
Schaalia odontolyticus
0 2 0 0 0
Schaalia meyeri h
0 2 5 0 0
Schaalia turicensis
u
31 99 81 0 98
Terrisporobacter glycolicus
0 88 0 0 0
Trueperella pyogenes
a
13 47 2 7 6
a Previously designated as Arcanobacterium pyo-
genes and prior to that Actinomyces pyogenes
0 33 0 0 0
b Previously designated as Lactobacillus minutis
86 90 5 0 0 C. botulinum Group I consists of toxin type A and
c
proteolytic strains of toxin type B and F.
86 90 5 0 0
C. botulinum Group II consists of toxin type E and
d
nonproteolytic strains of toxin type B and F.
0 92 0 0 0
Previously designated as Eubacterium aerofaciens.
e
Previously designated as Eubacterium lentum.
f
Previously designated as Propionibacterium
g
propionicum and prior to that Propionibacterium
33. Niles, A.C. and P.R. Murray. 1985. Abstract C-161. Abstracts
of the 85th General Meeting of the American Society for
Microbiology. ASM, Washington, D.C.
Spot
Indole
34. Ristow K.L., P.C. Schreckenberger, D.M. Celig, M.A. Ulanday,
ARG SER PYR IND and L.J. LeBeau. 1984. Abstract C-151. Abstracts of the 84th
General Meeting of the American Society for Microbiology.
V V V +
ASM, Washington, D.C.
35. Syed, S., W.J. Loesche, and C. Pearson. 1984. Abstract C-155.
+ + + + –
Abstracts of the 84th General Meeting of the American
Society for Microbiology. ASM, Washington, D.C.
– – – +
36. Clinical and Laboratory Standards Institute. 2008. Quality
Control for Commercial Microbial Identification Systems;
Approved Guideline. M50-A. CLSI, Wayne, PA.

18. SYMBOL LEGEND

RapID™ and ERIC™ are trademarks of Thermo Fisher Scientific
and its subsidiaries.
ATCC® is a registered trademark of American Type Culture
Collection.
www.thermofisher.com/microbiology
Tel: (800) 255-6730 • International: (913) 888-0939
www.oxoid.com/IFU
Europe +800 135 79 135 • US 1 855 2360 190
CA 1 855 805 8539 • ROW +31 20 794 7071
Version
IFU8311002
Printed in the UK
URE BLTS αARA ONPG αGLU βGLU αGAL αFUC NAG
0 0 0 0 0 12 0
0 29 61 87 99 97 93
90 15 0 96 86 96 93
81 76 0 91 99 92 62
0 18 2 78 99 0 99 12
0 0 0 0 0 26 99
0 79 87 90 99 87 92
0 88 0 87 16 76 93
0 42 0 51 99 98 79
0 0 0 0 82 26 0
c
0 0 0 0 99 0 0
d
0 9 85 91 98 0 99
0 0 0 0 0 0 0 94
0 84 90 84 85 87 98
0 2 0 2 8 3 0
0 0 1 0 0 0 0
0 5 0 0 20 36 5
0 0 0 0 0 0 0
0 51 2 99 9 94 5
0 39 69 98 76 36 96 18
0 90 0 84 99 99 76
0 2 16 99 14 0 2
0 0 0 7 39 32 0
0 0 0 2 0 5 0 36
0 84 76 98 47 26 96
0 0 0 0 0 0 0
0 77 9 2 76 10 5
0 0 0 5 52 0 0
0 0 5 0 82 0 67
n
0 0 0 0 3 0 0
k 0 5 0 9 99 99 5
0 0 0 0 0 7 0
0 0 0 9 0 0 0
r
0 0 0 0 0 0 0
0 81 0 21 91 99 12
j 0 89 0 70 78 96 0 22
0 26 2 96 86 0 99
l
0 71 1 0 82 99 2
0 19 0 12 99 5 95 11
0 26 2 31 99 5 0 15
98 0 0 0 26 0 0 42
t
0 0 0 0 2 0 0
o
0 4 15 86 96 46 5 92
i
0 2 0 69 93 0 0
0 0 0 0 99 0 0 28
0 0 0 10 0 16 0
q
0 12 15 86 0 0 0
propionicus
h Previously designated as Actinomyces meyeri
i Previously designated as Actinomyces odontol-
yticus
Previously designated as Lactobacillus casei
j
Previously designated as Lactobacillus catena-
k
forme
Previously designated as Lactobacillus fermentum
l
Previously designated as Propionibacterium acnes
m
Previously designated as Propionibacterium
n
granulosum
Catalogue Number
In Vitro Diagnostic Medical Device
Consult Instructions for Use (IFU)
Temperature Limitations (Storage temp.)
Contains sufficient for <N> tests
N
Do not use if package is damaged
Do not re-use
Batch Code (Lot Number)
Use By (Expiration Date)
Importer
Unique Device Identifier
Authorized representative in the European
Community
UK Conformity Assessed
European Conformity Assessment
Manufacturer
Remel Inc., 12076 Santa Fe Trail Drive, Lenexa, KS 66215, USA
Date of modifications introduced
August 2023
Updated to meet IVDR requirements
PO₄ LGY GLY PRO PAL ARG SER PYR
0 99 0 99 99 99 86 99 99 0
0 2 2 86 92 99 98 98 64 19
0 0 15 57 80 97 87 65 10 0
0 0 0 76 98 99 86 92 33 0
0 0 75 75 65 88 98 12 0
0 0 0 0 12 0 0 98 33 0
0 2 2 11 74 93 95 98 93 0
0 96 0 0 0 0 0 82 0 97
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 99 98 98 98 0
0 60 9 0 0 0 0 0 0 0
2 0 0 0 0 3 0 9 6 0
99 0 2 7 0 7 87 9 98
0 81 2 93 9 1 13 47 2 7
0 0 9 2 0 99 43 48 16 6
0 5 2 88 62 92 72 95 98 0
0 18 5 2 11 0 76 90 7 80
0 0 59 0 0 0 0 47 0 0
0 96 6 0 0 0 0 5 0 94
98 81 2 26 4 80 92 43 96
0 99 0 29 2 0 0 33 0 0
0 99 0 0 0 0 0 10 9 5
0 0 2 2 5 99 15 9 16 92
0 3 63 33 3 36 80 69 94
2 99 8 0 0 11 0 91 5 8
0 0 13 0 0 0 0 66 0 0
0 20 13 5 9 93 26 97 56 0
0 88 2 96 98 98 48 98 96 71
0 0 0 98 98 99 33 96 60 0
0 2 0 0 11 1 5 97 73 0
0 93 0 0 71 0 0 98 0 88
0 0 12 95 18 9 16 5 0 0
0 0 0 3 9 0 98 96 91 98
0 0 0 0 0 0 20 11 0 0
0 73 0 99 98 90 76 98 98 78
99 5 82 97 80 16 98 90 90
0 0 0 5 81 0 88 98 96 0
0 5 0 0 93 99 98 98 95 0
0 5 26 30 99 80 95 3 0
0 3 27 32 99 85 90 5 0
0 28 9 24 99 79 98 92 27
2 68 4 0 5 99 38 86 88 3
5 20 92 98 99 82 98 98 0
0 79 0 92 98 99 78 98 96 38
0 0 88 91 96 90 99 96 0
0 9 99 0 2 99 0 35 9 46
0 99 75 93 96 96 86 98 96 2
o Previously designated as Clostridium bifermentans
p Previously designated as Clostridium difficile
q Previously designated as Clostridium glycolicum
Previously designated as Clostridium histolyticum
r
Previously designated as Clostridium limosum
s
Previously designated as Clostridium sordellii
t
Previously designated as Actinomyces turicensis
u
AT02000B
IND
0
0
0
0
0
0
0
0
0
0
0
0
99
6
0
0
0
0
0
0
0
0
0
0
0
26
0
85
0
0
0
0
0
0
0
0
0
0
0
0
98
94
0
0
0
0
0